Neil Reiner, MD
Professor and Head
Division of Infectious Disease
Director
The Immunity and Infection Research Centre (IIRC)
phone: 604-875-4588
lab: 604-875-4348
fax: 604-875-4013
ethan(at)interchange.ubc.ca
www.id.med.ubc.ca/Faculty/Faculty_Reiner.htm
Host/Pathogen Interactions
My laboratory is concerned with host defense against intracellular infection and how intracellular microbes disrupt cellular functions to favor their survival. Because of our interest in the role of macrophages in host defense, one focus of the laboratory is to identify pathways that regulate cell activation is response to key agonists such as IFN-y and bacterial lipopolysaccharide. This research has led to the identification of novel signaling pathways that regulate macrophage function. The second major interest in the laboratory is to understand the strategies used by intracellular pathogens to effectively prevent macrophage activation. This research focuses on identifying the pathways and molecules in macrophages targeted by intracellular pathogens and the corresponding microbial virulence factors involved. Pathogens under study include, Leishmania donovani, M. tuberculosis and Salmonella.
Education
MD - Case-Western Reserve University
AB - Oberlin College
Selected Publications
Silverman JM, Chan SK, Robinson DP, Dwyer DM, Nandan D, Foster LJ, Reiner NE. Proteomic analysis of the secretome of Leishmania donovani. Genome Biol. 2008;9(2):R35.
Noubir S, Lee JS, Reiner NE. Pleiotropic effects of phosphatidylinositol 3-kinase in monocyte cell regulation. Prog Nucleic Acid Res Mol Biol. 2006;81:51-95.
Leon C, Nandan D, Lopez M, Moeenrezakhanlou A, Reiner NE. Annexin V associates with the IFN-gamma receptor and regulates IFN-gamma signaling. J Immunol. 2006 May 15;176(10):5934-42.
Cherkasov A, Lee SJ, Nandan D, Reiner NE. Large-scale survey for potentially targetable indels in bacterial and protozoan proteins. Proteins. 2006 Feb 1;62(2):371-80.
Reiner NE. Targeting cell regulation promotes pathogen survival in macrophages. Clin Immunol. 2005 Mar;114(3):213-5.
Cherkasov A, Nandan D, Reiner NE. Selective targeting of indel-inferred differences in spatial structures of highly homologous proteins. Proteins. 2005 Mar 1;58(4):950-4.
Hmama Z, Sendide K, Talal A, Garcia R, Dobos K, Reiner NE. Quantitative analysis of phagolysosome fusion in intact cells: inhibition by mycobacterial lipoarabinomannan and rescue by an 1alpha,25-dihydroxyvitamin D3-phosphoinositide 3-kinase pathway. J Cell Sci. 2004 Apr 15;117(Pt 10):2131-40.
Lee JS, Hmama Z, Mui A, Reiner NE. Stable gene silencing in human monocytic cell lines using lentiviral-delivered small interference RNA. Silencing of the p110alpha isoform of phosphoinositide 3-kinase reveals differential regulation of adherence induced by 1alpha,25-dihydroxycholecalciferol and bacterial lipopolysaccharide. J Biol Chem. 2004 Mar 5;279(10):9379-88.
Nandan D, Cherkasov A, Sabouti R, Yi T, Reiner NE. Molecular cloning, biochemical and structural analysis of elongation factor-1 alpha from Leishmania donovani: comparison with the mammalian homologue. Biochem Biophys Res Commun. 2003 Mar 21;302(4):646-52.
López M, Sly LM, Luu Y, Young D, Cooper H, Reiner NE. The 19-kDa Mycobacterium tuberculosis protein induces macrophage apoptosis through Toll-like receptor-2. J Immunol. 2003 Mar 1;170(5):2409-16.
Sly LM, Hingley-Wilson SM, Reiner NE, McMaster WR. Survival of Mycobacterium tuberculosis in host macrophages involves resistance to apoptosis dependent upon induction of antiapoptotic Bcl-2 family member Mcl-1. J Immunol. 2003 Jan 1;170(1):430-7.
Nandan D, Yi T, Lopez M, Lai C, Reiner NE. Leishmania EF-1alpha activates the Src homology 2 domain containing tyrosine phosphatase SHP-1 leading to macrophage deactivation. J Biol Chem. 2002 Dec 20;277(51):50190-7.
Sly LM, Guiney DG, Reiner NE. Salmonella enterica serovar Typhimurium periplasmic superoxide dismutases SodCI and SodCII are required for protection against the phagocyte oxidative burst. Infect Immun. 2002 Sep;70(9):5312-5.
Lesnick ML, Reiner NE, Fierer J, Guiney DG. The Salmonella spvB virulence gene encodes an enzyme that ADP-ribosylates actin and destabilizes the cytoskeleton of eukaryotic cells. Mol Microbiol. 2001 Mar;39(6):1464-70.
Nandan D, Knutson KL, Lo R, Reiner NE. Exploitation of host cell signaling machinery: activation of macrophage phosphotyrosine phosphatases as a novel mechanism of molecular microbial pathogenesis. J Leukoc Biol. 2000 Apr;67(4):464-70.
Hingley-Wilson SM, Sly LM, Reiner NE, McMaster WR. The immunobiology of the mycobacterial infected macrophage. Mod. Aspects of Immunobiol. 2000;1:96-101.
Sly LM, Lopez M, Nauseef WN, Reiner NE. 1a,25-Dihydroxyvitamin D3-induced monocyte antimycobacterial activity is regulated by phosphatidylinositol 3-kinase and mediated by the NADPH-dependent phagocyte oxidase, J. Biol. Chem. 2000;276:35482-35493.
Hmama Z, Nandan D, Sly L, Knutson KL, Herrera-Velit P, Reiner NE. 1alpha,25-dihydroxyvitamin D(3)-induced myeloid cell differentiation is regulated by a vitamin D receptor-phosphatidylinositol 3-kinase signaling complex. J Exp Med. 1999 Dec 6;190(11):1583-94.